概述

Papain is a sulfhydryl protease from Carica papaya latex. It has a molecular weight of 23 kDa and an optimum pH range of 6.0-7.0. The action of papain on leucine methyl ester produces an insoluble polyleucine peptide. Papain breaks down the intercellular matrix of cartilage. Papain is activated by cysteine, sulfide, and sulfite. Stabilizing agents are EDTA, cysteine and dimercaptoethanol.

技術規格

Stability	Stable for 6-12 months at 2-8°C. Do not freeze aqueous suspensions.
Technical Notes	Papain preparations should be incubated in the activation solution before use to ensure full activity. Applications include antibody fragmentation and primary/neural cell isolation.
Source	Carica Papaya Latex
Description	Supplied as a lyophilized powder prepared from a two times crystalline suspension. To insure full activity, the enzyme should be incubated in a solution containing 1.1mM EDTA, 0.067mM mercaptoethanol and 5.5mM cysteine-HCl for 30 minutes. It is recommended that the enzyme be 0.22 micron filtered after dissolution and prior to use.
Storage	Store at 2-8°C.
Activity	Activates to at least 15 units per mg protein
Unit Definition	One Unit hydrolyzes one micromole of benzoyl-L-arginine ethyl ester per minute at 25°C, pH 6.2, after activation in a solution containing 1.1 mM EDTA, 0.067 mM mercaptoethanol and 5.5 mM cysteine-HCl for 30 minutes.