產品描述
概述
Purified pectinase is a multi-component preparation highly effective in depolymerizing plant pectins with varying degrees of esterification. The product contains substantial hemicellulase, cellulase, pectinesterase and xylanase activities which together with pectin lyase and polygalacturonase work synergistically to digest plant cell wall tissues. When used with We purified cellulase, purified pectinase has been found to be highly successful for generating good yields of viable protoplasts in several plant systems, e.g., corn, soybean, red beet, sunflower, tomato and citrus. In general, a concentration range of 0.1% to 0.5% pectinase (with accompanying 0.5% to 1.5% cellulase) used at 24°C to 37°C for periods of 1 to 16 hours will yield good results.
技術規格
| Stability |
Protect from moisture. If not using entire bottle at once, weigh into single-use aliquots on arrival and store tightly covered and dessicated, at 2-8°C. Material is very hygroscopic and can become tacky and difficult to weigh if exposed to moisture. |
| Technical Notes |
Pectinase is extremely hydroscopic; store dessicated to protect from moisture. |
| Source |
Aspergillus niger |
| Description |
A chromatographically purified preparation also containing hemicellulase, cellulase, pectin-esterase and xylanase activities. Suitable for plant protoplast isolation applications. Supplied as a dialyzed, lyophilized powder. |
| Storage |
Store at 2-8°C. PROTECT FROM MOISTURE. |
| Activity |
≥20 units per mg dry weight |
| Unit Definition |
One Unit releases 1μmole of D-galacturonic acid from polygalacturonic acid per minute at 37°C, pH 5.0. |
