概述

Tyrosine decarboxylase catalyzes the removal of the carboxyl group from tyrosine to produce tyramine and carbon dioxide. Pyridoxal 5'-phosphate is a necessary cofactor. By using the apoenzyme prepared from cells grown on a vitamin B6 deficient medium pyridoxal phosphate may be determined. The HOLOenzyme may be used to determine tyrosine, phenylalanine and dihydroxyphenylalanine either manometrically or colorimetrically.

技術規格

Source	Streptococcus faecalis (NCTC6783)
Description	APOenzyme. Dried cells grown in B6 deficient media.
Storage	Store at -20°C.
REQUIRES SPECIAL SHIPPING	DRY ICE
Activity	Activates to at least 0.2 units per mg
Unit Definitions	One Unit yields 1μmole of CO2per minute from L-tyrosine at 37°C, pH 5.5. The APOenzyme activity is measured in the presence of excess pyridoxal phosphate.