Order NO. B002138
CAS :[9003-98-9]
脫氧核糖核酸酶 I
| 英文名 | Deoxyribonuclease I | ||
| 級別 | Activity ≥2,000 Kunitz U/mg dw | 相關類別 | 必威betway官方网站登录 |
| 儲存 | 冷藏（2-8℃） 幹燥 | EINECS編號 | 232-667-0 |
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| 編 號 | 包裝 | 庫存 | 目錄價（￥） | 您的價格（￥） | 數 量 |
| B002138-0001 | 1 G | 預計90日內發貨 |  |
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| B002138-0025 | 25 MG | 現貨 | |
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| B002138-0100 | 100 MG | 現貨 | |
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產品描述
概述
Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.
技術規格
| Uses | Applications have included nick translation, DNA mapping, isolation of nuclear RNA and protein, plasmid construction, and RNA polymerase synthesis of RNA probes and RT-PCR. DNase is also used in tissue culture work to digest DNA from damaged cells thereby reducing viscosity, and removing membrane bound DNA fragments. |
| Stability | When properly stored, this product is stable for 2-3 years. Recommended storage temperature is 2-8°C. |
| Source | Bovine Pancreas |
| Description | Partially purified. A lyophilized powder. |
| Storage | Store at 2-8°C. |
| Activity | ≥2,000 Kunitz units per mg dry weight |
| Unit Definition | 1 Unit causes an increase in absorbance at 260 nm of 0.001 per minute per ml at 25°C when acting upon highly polymerized DNA at pH 5.0.Note: Kunitz units as reported by other suppliers can be 2 to 4 times higher than Kunitz units as measured at our labs. As measured at at our labs, one Kunitz unit digests 1μg of lambda DNA in 10 minutes at 37°C in 50mM Tris, 1mM Mg2+, 1mM Ca2+, pH 7.8 in a 50μl reaction. Correlation of digestion units with Kunitz units is different for other DNA and buffer systems. |
相關文檔
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安全信息
質量控製相關文件
質量報告 (COA)