Order NO. B003602
CAS :[9068-54-6]
磷酸二酯酶 II
| 英文名 | Phosphodiesterase II | ||
| 級別 | Activity ≥1.2 U/mg dw | 相關類別 | 必威betway官方网站登录 |
| 儲存 | 冷凍（-20℃） 避光+幹燥 | EINECS編號 | 232-965-0 |
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| 編 號 | 包裝 | 庫存 | 目錄價（￥） | 您的價格（￥） | 數 量 |
| B003602-0025 | 25 U | 預計40日內發貨 |  |
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產品描述
概述
Spleen exonuclease (Phosphodiesterase II) excises 3'-phosphomononucleotides from oligonucleotides having a free OH terminus. The optimum pH for the enzyme is 5.5 using succinate and phosphate buffer and pH 6-7 with 0.1 M acetate buffer. The enzyme is assayed using a modification of the Hilmoe (Biochem. Prep., VII, (Meister, A., ed.), John Wiley and Sons, NY, 105, 1961) technique.
技術規格
| Source | Bovine Spleen |
| Description | Prepared from the 1mM sodium pyrophosphate, pH 6.9, alumina gel eluate of Hilmoe:Biochem. Prep VIII(Meister, A., ed.), John Wiley & Sons, NY, NY, p. 105 (1961). Lyophilized in vials. |
| Storage | Store at -20°C. |
| Activity | ≥1.2 u/mg dw |
| Unit Definition | One Unit increases the absorbance at 260 nm by 0.200 in 30 minutes at 37°C, pH 6.5, with an RNA substrate. |
相關文檔
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安全信息
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