Order NO. B005410
CAS :[9025-64-3]
脫氧核糖核酸酶 II，已純化
| 英文名 | Deoxyribonuclease II, Purified | ||
| 別名 | Deoxyribonuclease II | ||
| 級別 | Activity ≥12,000 U/mg protein | 相關類別 | 必威betway官方网站登录 |
| 儲存 | 冷凍（-20℃） | EINECS編號 | 232-801-8 |
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| 編 號 | 包裝 | 庫存 | 目錄價（￥） | 您的價格（￥） | 數 量 |
| B005410-0020 | 20 KU | 預計40日內發貨 |  |
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產品描述
概述
Deoxyribonuclease II from porcine spleen has a molecular weight of 38 kDa. The enzyme is a glycoprotein endonuclease with dimeric structure. Optimum pH range is 4.5-5.0 at ionic strength 0.15 M. Deoxyribonuclease II (Acid DNase) hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. It also acts on p-nitrophenylphosphodiesters at pH 5.6-5.9. Bernardi (BBRC, 17, 573, 1971) describes a three stage degradation of native DNA by DNase II.
技術規格
| Source | Porcine Spleen |
| Description | Chromatographically purified in a modification of the procedure of Bernardi,et al., BBA, 129,1 (1966). A dialyzed, lyophilized powder. |
| Storage | Store at -20°C. |
| REQUIRES SPECIAL SHIPPING | ICE PACK |
| Activity | ≥12,000 units per mg protein |
| Unit Definition | One Unit causes an increase in absorbance at 260 nm of 0.001 per minute at 25°C, pH 4.6 using highly polymerized DNA as substrate. |
相關文檔
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安全信息
| WGK 德國 | 9999999 |
質量控製相關文件
質量報告 (COA)