概述

The SDM32 ReallBand 10 KB Plus (0.1-10 kb) DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct loading on gel. The DNA Ladder SDM32 is composed of 19 individual DNA fragments: 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200, and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains three enhanced bands (3 kb, 1.5 kb and 500 bp) for easy referencing. In addition, three tracking dyes, Xylene cyanol FF, Bromophenol blue and Orange G which mimic the migration of 4,000 bp, 500 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring.

使用說明

5 μl per well for 100 gel applications

技術規格

Storage	Protected from light, Room temperature ≥ 6 months; 4°C ≥ 12 months; -20°C ≥ 24 months
Buffering	Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Concentration	86 μg/ 500 μl