概述

This kit is designed for the coupling of peptides to carrier proteins, utilizing the primary amines and carboxyl groups of the peptides and carrier proteins. This kit utilizes the chemical heterobifunctional crosslinker EDC (1-Ethyl-3-(6-dimethylaminopropyl) carbodiimide hydrochloride) to couple peptides to carrier proteins. EDC first reacts with the carboxyl groups, producing an amine reactive intermediate, O-acylisourea that rapidly reacts with the amine groups of the peptide/protein. This kit utilizes Tube-O-Reactor(TM), which allows for the reactions to be performed in a single tube, with no loss of essential reagents and minimum hands on time and effort.

特點

• Simple to use, single step reaction; Suitable for all carrier proteins; Uses single tube reactor for minimum hands on time.

應用

Application for coupling of peptides to carrier proteins;^Suitable for antibody production.

組份

5X Optimizer Buffer IV;EDC (1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride), 50mg/vial ;Tube-O-Reactor (Medi) includes 5 Tube-O-Dialyzers (MWCO 8000) 5 floats, 5 Dialysis Cups and glass balls;OneQuant Keyhole Limpet Hemocyanin (2mg/vial)