概述

Perfect-FOCUS(TM) is designed to clean and concentrate protein samples that give poor protein spot resolution during 2D electrophoresis. Protein samples containing interfering agents, including ionic detergents, metal ions, substrates, substrate analogs, inhibitors, and other charged molecules, routinely result in smeared or low resolution gels. Treated samples are compatible with mass spectrometry analysis and show identical peptide spectra.

特點

• Conductivity lower than 50 μS;
• Samples free from non-protein interfering agents;
• Greatly reduced spot streaking and improved spot resolution;
• Perfect protein migration patterns;
• Suitable for running 2D gels and compatible with mass spectrometry analysis;
• Process up to 50 protein samples (or 6 with the trial size kit), 1-100μl/each.

應用

Prepare low conductivity samples for IEF (isoelectric focusing) and 2D gel electrophoresis applications;^Concentrate and clean protein samples;^Remove interfering agents from protein solutions;^Improve protein spot resolution and prepare streak-free 2D gels.

組份

UPPA-I; UPPA-II; FOCUS(TM)-Wash; OrgoSol Buffer; SEED; Perfect-FOCUS(TM) Buffer-I; Perfect-FOCUS(TM) Buffer-II