概述

The kit has been specifically developed for concentration of those proteins that cannot be concentrated either by precipitation or other techniques. The kit is based on a special Protein Binding Resin that binds and immobilizes any protein in a low salt buffer between pH 2~12 (capacity ~ 0.5 mg protein/ml Protein Binding Resin). The immobilized protein is spin-eluted in a small volume of specifically formulated elution buffer, giving several fold effective concentration. This method is gentle and protects protein from denaturation during the concentration process. The kit has been tested for concentration of a wide variety of proteins up to 5 mg in either single or multiple procedure and supplied with a proprietary spin column that may be regenerated and re-used once. Suitable for larger volumes of dilute protein solution.

應用

Concentration of dilute protein solution

組份

Protein Binding Resin; Protein Elution Buffer [2X]; Spin Columns, empty; SpinOUT, GT-600 (Medi); Collection Tubes