概述

The first section of the protocol covers the in-gel digestion of proteins by our chemically modified Mass Spectrometry Grade Trypsin. Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues. Typically there is little or no cleavage at arginyl-proline and lysyl- proline bonds. Trypsin naturally undergoes autolysis, producing trypsin fragments that interfere with sequence analysis. Our Mass Spectrometry Grade Trypsin is a chemically modified trypsin that is enzymatically active and yet resistant to autolysis. It is methylated, TPCK treated, affinity purified and quality tested for mass spectrometry. The second part of the protocol focus on the extraction of the digested proteins from the gel pieces for analysis by mass spectrometry (MALDI and LC MS/MS). The InGel ? Silver kit is suitable for ~100 protein spots or bands.

特點

• The InGel ? Silver kit provides a complete set of reagents for the in gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS).

應用

The kit is specifically designed for use with silver stained protein spots/bands.

組份

SilverOUT? I，SilverOUT? II，Trypsin Digestion Buffer，Pep-Extract?，Trypsin, Mass Spectrometry Grade，Trypsin Suspension Buffer，OneQuant DTT，OneQuant Iodoacetamide