概述

Iodoacetyl-LC-Biotin is long-chain, sulfhydryl-reactive biotinylation reagents.For the reagent,reaction of the iodoacety1 group with a sulfhydryl (-SH) group is rapid and specific, especially when only a slight reagent-to-sulfhydrylmolar excess is used and the reaction is performed at pH 8.3 (7.5-8.5).1-3 The reaction occurs by nucleophilic substitution of iodine with a thiol (sulfhydryl) group, resulting in a stable thioether bond (Figure 1). If thiols are unavailable on the molecule, reaction can occur with histidy1 side chains at pH 6.9-7.0, but the reaction must be allowed to proceed for at least one week. Iodoacetyl reagents also will react with amino groups at pH > 10.

應用

Iodoacetyl-LC-Biotin is not soluble in water and must be dissolved in an organic solvent such as DMF before further dilution in aqueous solutions.

組份

50 mg/bottle

技術規格

Formula	C18H31IN4O3S
Net Mass Addition	382.53
Spacer arm	27.1
Purity	>95% on TLC
Solubility	> 2 mg/ml in DMF for subsequent dilution in water or buffer