概述

Biotin-BMCC is a sulfhydryl-reactive biotinylation reagent with a long spacer arm. Maleimide-activated reagents are effective for protein modification of sulfhydryl groups. Maleimide groups react efficiently and specifically with free (reduced) sulfhydryls at pH 6.5-7.5 to form stable thioether bonds (Figure 1). Most proteins have cysteine residues whoseside-chain sulfur atoms typically occur in pairs as disulfide bonds. Reduction of these disulfide bonds exposes the sulfhydryl group required as a target for biotinylation with maleimide-activated reagents. Alternatively, sulfhydryl groups can be addedto molecules using various modification reagents (see subsequent Important Product Information Section).

應用

Biotin-BMCC is not directly soluble in water or aqueous buffers; however, if it is first dissolved in dimethylsulfoxide (DMSO) or other organic solvent, then it can be diluted and remain soluble in an aqueous buffer at typical reaction concentrations.

組份

50 mg/bottle

技術規格

Molecular Weight	533.68
Spacer arm	32.6
Purity	>95% on TCL
Reaction pH	acidic or neutral pH
Solubility	~4.5 mg/ml (8.5 mM) in DMSO (slightly less soluble in DMF)