概述

SUMO-Conjugating Enzyme UBC9 belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2I gene in humans. The ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, take part in the second step in the ubiquitination reaction. In this reaction, E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue. The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region. The UBC9 accepts the ubiquitin-like proteins SUMO1-4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Additionally, It takes parts in catalysis the formation of poly-SUMO chains, sumoylation of FOXL2 and KAT5, and the segregation of nuclear architecture and chromosome.

使用說明

This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

技術規格

Physical Appearance	Sterile liquid.
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Source	Escherichia coli.
Biological Activity	Data is not available.
Formulation	50 mM HEPES ,125 mM NaCl, 1 mM DTT,10% glycerol, pH 7.6.
Endotoxin	Less than 1 EU/μg of rHuUBC9, His as determined by LAL method.
Reconstitution	Further dilutions should be made in appropriate buffered solutions.
AA Sequence	MHHHHHHAMG TLNMSGIALS RLAQERKAWR KDHPFGFVAV PTKNPDGTMN LMNWECAIPG KKGTPWEGGL FKLRMLFKDD YPSSPPKCKF EPPLFHPNVY PSGTVCLSIL EEDKDWRPAI TIKQILLGIQ ELLNEPNIQD PAQAEAYTIY CQNRVEYEKR VRAQAKKFAP S