概述

Ubiquitin-conjugating enzyme E2 R1 belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2R1 gene in humans. The ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, take part in the second step in the ubiquitination reaction. In this reaction, E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue. The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region. UBE2R1 is important in the control of cell cycle and DNA replication. In vitro it catalyzes Lys-48-linked polyubiquitination. Additionally, the protein cooperates with the UBCH5C and the SCF ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation.

使用說明

This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

技術規格

Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Source	Escherichia coli.
Biological Activity	Data is not available.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, 1mM DTT, pH 7.5.
Endotoxin	Less than 0.1 EU/μg of rHuUBE2R1 as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
AA Sequence	MHHHHHHAMG ILMARPLVPS SQKALLLELK GLQEEPVEGF RVTLVDEGDL YNWEVAIFGP PNTYYEGGYF KARLKFPIDY PYSPPAFRFL TKMWHPNIYE TGDVCISILH PPVDDPQSGE LPSERWNPTQ NVRTILLSVI SLLNEPNTFS PANVDASVMY RKWKESKGKD REYTDIIRKQ VLGTKVDAER DGVKVPTTLA EYCVKTKAPA PDEGSDLFYD DYYEDGEVEE EA