概述

Interferon- gamma (IFN-γ), also known as Type II interferon or immune interferon, is a cytokine produced primarily by T -lymphocytes and natural killer cells. The protein shares no significant homology with IFN-β or the various IFN- α family proteins. Mature IFN-γ exists as noncovalently-linked homodimers. IFN-γ was originally characterized based on its antiviral activities. The protein also exerts antiproliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanis ms. IFN-γ induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. Additionally, IFN-γ augments TH1 cell expansion and may be required for TH1 cell differentiation. Equine IFN-γ shares 73%~ 82% amino acid sequence identity with bovine, canine, feline, and porcine IFN-γ and 42 %~64 % with cotton rat, human, murine, rat, and rhesus macaque IFN-γ .

使用說明

This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

技術規格

Physical Appearance	Sterile Filtered White lyophilized (freeze- dried) powder.
Purity	> 97 % by SDS-PAGE and HPLC analyses.
Source	Escherichia coli.
Biological Activity	Fully biologically active when compared to standard. The ED50 as determined by an anti - viral assay using human HeLa cells infected w ith encephalomyocarditis (EMC) virus is less than 10.0 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg.
Formulation	Lyophilized from a 0.2 μ m filtered concen trated solution in 2 × PBS, pH 7.4, with 5 % trehalose .
Endotoxin	Less than 0. 1 EU/ μ g of rEqIFN -γ as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
AA Sequence	QAAFFKEIEN LKEYFNASNP DVGDGGPLFL DILKNWKEDS DKKIIQSQIV SFYFKLFENL KDNQVIQKSM DTIKEDLFVK FFNSSTSKLE DFQK LIQIPV NDLKVQRKAI SELIKVMNDL SPKANLRKRK RSQNPFRGRR ALQ