Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members.
Applications:
WB
Name of antibody:
PRKCA(Phospho-Tyr657)
Immunogen:
Peptide sequence around phosphorylation site of tyrosine 657(F-S-Y(p)-V-N) derived from Human PKC α.
Full name:
protein kinase C, alpha
Synonyms:
AAG6; PKCA; PRKACA; PKC-alpha
SwissProt:
P17252
WB Predicted band size:
77 kDa
WB Positive control:
COLO205 lysates
WB Recommended dilution:
500-1000
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Sample Application
Gel: 10%SDS-PAGE, Lysate: 30 μg, Lane 1-2: COLO205 lysates, lane2 is treated with the antigen-specific peptide , Primary antibody: D151256(PRKCA(Phospho-Tyr657) Antibody) at dilution 1/500 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute