This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex.
Applications:
WB, IHC, IF
Name of antibody:
MUC1(Phospho-Tyr1229)
Immunogen:
Peptide sequence around phosphorylation site of tyrosine 1229 S-P-Y(p)-E-K) derived from Human CD227/MUC1.
The image on the left is immunohistochemistry of paraffin-embedded Human breast carcinoma tissue using D151275(MUC1(Phospho-Tyr1229) Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)
Gel: 8%SDS-PAGE, Lysate: 30 μg, Lane 1-2: HepG2 cells lysates treated with PMA, lane2 is treated with the antigen-specific peptide , Primary antibody: D151275(MUC1(Phospho-Tyr1229) Antibody) at dilution 1/500 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
The image is immunofluorescence of HepG2 cells using D151275(MUC1(Phospho-Tyr1229) Antibody) at dilution 1/100.