Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells.
Applications:
WB
Name of antibody:
PRKCD(Phospho-Tyr64)
Immunogen:
Peptide sequence around phosphorylation site of Tyrosine 64 (H-I-Y(p)-E-G) derived from Human PKCD.
Full name:
protein kinase C, delta
Synonyms:
MAY1; PKCD; CVID9; nPKC-delta
SwissProt:
Q05655
WB Predicted band size:
78 kDa
WB Positive control:
HepG2 cells lysates treated with PMA
WB Recommended dilution:
500-1000
相關文檔
Sample Application
Gel: 10%SDS-PAGE, Lysate: 30 μg, Lane 1-2: HepG2 cells lysates treated with PMA, lane2 is treated with the antigen-specific peptide , Primary antibody: D151423(PRKCD(Phospho-Tyr64) Antibody) at dilution 1/500 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute