Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
Applications:
WB
Name of antibody:
VCL(Phospho-Tyr822)
Immunogen:
Peptide sequence around phosphorylation site of Tyrosine 822 (S-G-Y(p)-R-I) derived from Human Vinculin.
Full name:
vinculin
Synonyms:
MV; MVCL; CMD1W; CMH15; HEL114
SwissProt:
P18206
WB Predicted band size:
124 kDa
WB Positive control:
Hela cells lysates treated with Forskolin
WB Recommended dilution:
500-1000
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Sample Application
Gel: 8%SDS-PAGE, Lysate: 30 μg, Lane 1-2: Hela cells lysates treated with Forskolin, lane2 is treated with the antigen-specific peptide , Primary antibody: D151472(VCL(Phospho-Tyr822) antibody) at dilution 1/500 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute