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Order NO. D151616
兔抗AKT1(Ab-129) 多克隆抗體
英文名 Anti-AKT1(Ab-129) rabbit polyclonal antibody
別名 AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
相關類別 一抗 儲存 冷凍(-20℃) 避光
宿主 Rabbit 反應種屬 Human
標記物 Unconjugate 克隆類型 rabbit polyclonal
抗原 AKT1(Ab-129)
編 號 包裝 庫存 目錄價(¥) 您的價格(¥) 數 量
D151616-0025 25 ul 現貨
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D151616-0100 100 ul 預計7日內發貨
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D151616-0200 200 ul 預計7日內發貨
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技術規格

Background: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase.
Applications: WB, IHC, IF
Name of antibody: AKT1(Ab-129)
Immunogen: Synthesized non-phosphopeptide derived from human Akt around the phosphorylation site of serine 129 (D-N-S(p)-G-A).
Full name: v-akt murine thymoma viral oncogene homolog 1
Synonyms: AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
SwissProt: P31749
IHC positive control: Human skeletal muscle tissue
IHC Recommend dilution: 50-100
WB Predicted band size: 56 kDa
WB Positive control: Huvec cells treated with Serum (30%, 30mins) lysate
WB Recommended dilution: 500-3000
IF positive control: Hela cells
IF Recommend dilution: 100-500
相關文檔
Sample Application

  • The image on the left is immunohistochemistry of paraffin-embedded Human skeletal muscle tissue using D151616(AKT1(Ab-129) Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)


  • Gel: 10%SDS-PAGE, Lysate: 30 μg, Lane: Huvec cells treated with Serum (30%, 30mins) lysate, Primary antibody: D151616(AKT1(Ab-129) Antibody) at dilution 1/500 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute

  • The image is immunofluorescence of Hela cells using D151616(AKT1(Ab-129) Antibody) at dilution 1/100.
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