The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein.
Applications:
WB, IHC
Name of antibody:
STAT4 (Phospho-Tyr693)
Immunogen:
Synthetic peptide of human STAT4 (Phospho-Tyr693)
Full name:
signal transducer and activator of transcription 4 (Phospho-Tyr693)
Synonyms:
SLEB11
SwissProt:
Q14765
IHC positive control:
Human breast carcinoma
IHC Recommend dilution:
50-100
WB Predicted band size:
86 kDa
WB Positive control:
HepG2 cells treated with IL-4
WB Recommended dilution:
500-1000
相關文檔
Sample Application
The image on the left is immunohistochemistry of paraffin-embedded Human breast carcinoma tissue using D155019(STAT4 (Phospho-Tyr693) Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)
Gel: 8%SDS-PAGE, Lysate: 30 μg, Lane 1-2: HepG2 cells treated with IL-4, HepG2 cells untreated with IL-4, Primary antibody: D155019(STAT4 (Phospho-Tyr693) Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute