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Order NO. D199442
小鼠抗TP53單克隆抗體
英文名 Anti-TP53 mouse monoclonal antibody
別名 BCC7; LFS1; P53; TRP53
相關類別 一抗 儲存 冷凍(-20℃) 避光
宿主 Mouse 反應種屬 Human , Monkey , Mouse
標記物 Unconjugate 克隆類型 mouse monoclonal
抗原 TP53
編 號 包裝 庫存 目錄價(¥) 您的價格(¥) 數 量
D199442-0100 100 ul 預計5日內發貨
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技術規格

Background: This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]
Applications: WB, IHC, IF, FC
Name of antibody: TP53
Immunogen: Fusion protein of human TP53
Full name: tumor protein p53
Synonyms: P53; BCC7; LFS1; BMFS5; TRP53
SwissProt: P04637
IHC positive control: carcinoma of human lung tissue and adenocarcinoma of human ovary tissue; carcinoma of human pancreas tissue
IHC Recommend dilution: 30-150
WB Predicted band size: 44 kDa
WB Positive control: Hela, SVT2, COS7 cell lysates
WB Recommended dilution: 500-2000
IF Positive control: COS7 cells transiently transfected by pCMV6-ENTRY
IF Recommended dilution: 100-200
FC Recommend dilution: 50-100
相關文檔
Sample Application

  • The image is flow cytometry analysis using D199442(TP53 antibody) at dilution 1/100, staining Hela cells(Red), compared to a nonspecific negative control antibody(Blue)

  • The image is immunofluorescence of COS7 cells transiently transfected by pCMV6-ENTRY using D199442(TP53 Antibody) at dilution 1/100

  • The image on the left is immunohistochemistry of paraffin-embedded carcinoma of human lung tissue, on the right is immunohistochemistry of paraffin-embedded adenocarcinoma of human ovary tissue using D199442(TP53 Antibody) at dilution 1/40. (Original magnification: ×200)


  • The image is immunohistochemistry of paraffin-embedded carcinoma of human pancreas tissue using D199442(TP53 Antibody) at dilution 1/40. (Original magnification: ×200)

  • Gel: 10%SDS-PAGE, Lysate: 35μg, Lane 1-9: HepG2, Hela, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF-7 cell lysates, Primary antibody: D199442(TP53 Antibody) at dilution 1/500, Secondary antibody: Goat anti mouse IgG at 1/8000 dilution, Exposure time: 15 minutes
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