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Order NO. D224513
兔抗CEP72多克隆抗體
英文名 Anti-CEP72 rabbit polyclonal antibody
別名 centrosomal protein 72;
相關類別 一抗 儲存 冷凍(-20℃)
宿主 Rabbit 反應種屬 Human, Mouse
標記物 Unconjugate 克隆類型 rabbit polyclonal
抗原 CEP72
編 號 包裝 庫存 目錄價(¥) 您的價格(¥) 數 量
D224513-0025 25 ul 現貨
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D224513-0100 100 ul 現貨
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D224513-0200 200 ul 現貨
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技術規格

Background: Centrosomes are the major microtubule-organizing centers of mammalian cells. They are composed of a centriole pair and surrounding microtubule-nucleating material termed pericentriolar material (PCM). Bipolar mitotic spindle assembly relies on two intertwined processes: centriole duplication and centrosome maturation. Failure to properly orchestrate centrosome duplication and maturation is subsequently linked to spindle defects, which can result in aneuploidy and promote cancer progression. CEP72 (centrosomal protein 72kDa) is a 647 amino acid protein that localizes to the centrosome and centrosome-surrounding particles throughout the cell cycle. Involved in the recruitment of key centrosomal proteins to the centrosome, CEP72 provides centrosomal microtubule-nucleation activity on the Tubulin ring complexes and has critical roles in forming a focused bipolar spindle, which is needed for proper tension generation between sister chromatids. CEP72 exists as two alternatively spliced isoforms.
Applications: ELISA, WB, IHC
Name of antibody: CEP72
Immunogen: Fusion protein of human CEP72
Full name: centrosomal protein 72
SwissProt: Q9P209
ELISA Recommended dilution: 5000-10000
IHC positive control: Human esophagus cancer and Human liver cancer
IHC Recommend dilution: 30-150
WB Predicted band size: 72 kDa
WB Positive control: TM4, NIH/3T3 and Jurkat cell lysates
WB Recommended dilution: 200-1000
相關文檔
Sample Application

  • The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using D224513(CEP72 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)


  • The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using D224513(CEP72 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)


  • Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-3: TM4,NIH/3T3 and Jurkat cell lysates, Primary antibody: D224513(CEP72 Antibody) at dilution 1/600, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
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