應用

用於人血清、血漿或其他相關生物液體中Ras相關C3肉毒菌毒素底物1的測定。
For the determination of Human Rac1(Ras Related C3 Botulinum Toxin Substrate 1) concentrations in serum, plasma and other related biological fluids.

技術規格

工作原理	本試劑盒采用的是雙抗體夾心法酶聯免疫吸附檢測技術（ELISA）。測定樣品中人Ras相關C3肉毒菌毒素底物1水平。向預先包被了抗人Ras相關C3肉毒菌毒素底物1抗體的酶標孔中，加入標準品和樣本，溫育後，加入生物素標記的抗Ras相關C3肉毒菌毒素底物1抗體。再與HRP標記的鏈黴親和素結合，形成免疫複合物，再經過溫育和洗滌，去除未結合的酶，然後加入顯色底物TMB，產生藍色，並在酸的作用下轉化成最終的黃色。最後，在450 nm處測定反應孔樣品吸光度（OD）值，樣本中的人Ras相關C3肉毒菌毒素底物1濃度與OD值成正比，通過繪製標準曲線計算出樣本中人Ras相關C3肉毒菌毒素底物1的濃度。
Purpose	This assay employs the Sandwich enzyme immunoassay technique. Determine the level of Human Rac1(Ras Related C3 Botulinum Toxin Substrate 1) in the sample. Add standard and sample to the microplate that have been pre-coated with Anti-Human Rac1(Ras Related C3 Botulinum Toxin Substrate 1) antibody. After incubation, add Biotin-conjugated anti-Human Rac1(Ras Related C3 Botulinum Toxin Substrate 1) antibody. It is then combined with HRP-conjugated streptavidin to form an immune complex, then incubated and washed to remove unbound enzyme, and then added to the chromogenic substrate TMB to produce a blue color, and converted to the final yellow under the action of acid. Finally, the absorbance (OD) value was measured at 450 nm. The concentration of Human Rac1(Ras Related C3 Botulinum Toxin Substrate 1) in the sample was proportional to the OD value. The concentration of Human Rac1(Ras Related C3 Botulinum Toxin Substrate 1) in the sample can be calculated by drawing a standard curve.
靈敏度	0.1 ng/mL
Sensitivity	0.1 ng/mL
檢測範圍	0.16-10 ng/mL
Examination range	0.16-10 ng/mL
特異性	可檢測樣本中的人Ras相關C3肉毒菌毒素底物1，且與其類似物無明顯交叉反應。
Specificity	This kit recognizes Human Rac1(Ras Related C3 Botulinum Toxin Substrate 1) in samples. No significant cross-reactivity or interference between Human Rac1(Ras Related C3 Botulinum Toxin Substrate 1) and analogues was observed.
重複性	三種不同濃度的樣本在同一塊板子上分別檢測20次，以及在不同的板子上分別檢測20次，板內和板間變異係數均小於10%。
Repeatability	Three different concentrations of samples were detected 20 times on the same board and 20 times on different boards. Within the plate or between the plates, the coefficient of variation is <10%.
回收率	分別往稀釋不同倍數的5個樣本中加入已知濃度的目標蛋白，做回收實驗，得出回收率範圍和平均回收率。
Recovery	A known concentration of the target protein was added to 5 samples which were diluted to different multiples, and a recovery experiment was performed to obtain a recovery range and an average recovery rate.