The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.
Applications:
WB
Name of antibody:
EZR(Phospho-Tyr478)
Immunogen:
Peptide sequence around phosphorylation site of tyrosine 478 (P-V-Y(p)-E-P) derived from Human Ezrin.
Full name:
ezrin
Synonyms:
CVL; CVIL; VIL2; HEL-S-105
SwissProt:
P15311
WB Predicted band size:
69 kDa
WB Positive control:
K562 cells lysates treated with Na3VO4
WB Recommended dilution:
500-1000
相關文檔
Sample Application
Gel: 10%SDS-PAGE, Lysate: 30 μg, Lane 1-2: K562 cells lysates treated with Na3VO4, lane2 is treated with the antigen-specific peptide , Primary antibody: D151410(EZR(Phospho-Tyr478) Antibody) at dilution 1/500 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute