CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated.
Applications:
WB
Name of antibody:
CDC25A(Phospho-Thr507)
Immunogen:
Peptide sequence around phosphorylation site of Threonine 507
(S-R-T(p)-W-A) derived from Human CDC25A.
Full name:
cell division cycle 25A
Synonyms:
CDC25A2
SwissProt:
P30304
WB Predicted band size:
59 kDa
WB Positive control:
Huvec cells lysates
WB Recommended dilution:
500-1000
相關文檔
Sample Application
Gel: 10%SDS-PAGE, Lysate: 30 μg, Lane 1-2: Huvec cells lysates, lane1 is treated with the antigen-specific peptide , Primary antibody: D151574(CDC25A(Phospho-Thr507) Antibody) at dilution 1/500 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute